JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102162.1
Database:	nt	Go to original record
Description:	Maianthemum japonicum isolate P372 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Maianthemum japonicum
Original size:	973 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	545	+	535 bp

Amplicon:

535 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aacgaaaaat
101   gggcaatcct gagccaaatc tttattttta gaaaaacaag cttttttctt
151   ttaaaaacta gaaaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgacta cgttgcattg gtaactagaa tccttctaaa
251   ttaaagataa attaaggaga ggatgaccta tatatctaat acgtacgtat
301   acatactagc agattaatca cgacctgaat tcattgttga aggaaaaatt
351   gaatattcag tgatcaaatc attcattcca gagtttgata gaccttcttt
401   tgaaaaactg attaatcgga cgagaataaa gagagagtcc cattctacat
451   gtcaataccg acaacaatga aatttatagt aagaggaaaa tccgtcgact
501   ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	535	516	2.41e-08

Match details