JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102161.1
Database:	nt	Go to original record
Description:	Chrysosplenium japonicum isolate P307 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Chrysosplenium japonicum
Original size:	809 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	549	+	539 bp

Amplicon:

539 bp

1     cgaaatcggt agacgctacg gacttaattg agttgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatttaaaat
101   gggcaatcct gagccaaatc ctgttttacg aaaacaaaac acaaaggttc
151   agaaaaagag aataaaaaaa agggataggt gcagagactc aatggaagct
201   attctaacaa aaggacttga ctgcggtgcg ttggaaaagg aatccttcca
251   tcgaaacttc agaaaggctt caaatgataa tgatgactcg aacccgtatt
301   ttttatttga aaaaagtaca aataattgtt gtaaattgat tccaagttga
351   ataaaaaatc aaatattcat tgataaaata attcacttca tagtctgata
401   aattttttga agagcccatt aagacgagaa taaagataga gtcccattct
451   acatgtcaat gcagacagca acgaaattta tagtaagagg aaaatccgtc
501   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	539	520	2.42e-08

Match details