JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102160.1
Database:	nt	Go to original record
Description:	Cardamine violifolia isolate P303 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cardamine circaeoides
Original size:	1176 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	597	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc cttgtttacg cgaacaaacc tgagtttaga
151   aagcgagaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatgaagtt cactaccttg tgttgataaa ggaatccttc gatcgaaact
251   tcaaatcaaa aaggatgaag gagaaaagcc tatattgtct aaatataggt
301   aacacaaaac gatctcaaaa atgacgacct gaatcccgat ttcttttttt
351   tataaacaaa atagaaatgt tgtgaatcaa ttcgaagttt aagaaaaaat
401   caaatattca ttgatcaaat aattcacttc atgcatagtc tgatagatcc
451   ttggtggaac tttttaatcg gacgagaata aagatagagt cccattctac
501   atgtcaatac tgacaacaat gaaatttata gtaagatgaa aatccgttga
551   ctttttaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details