JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102158.1
Database:	nt	Go to original record
Description:	Stephanandra incisa isolate P197 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Neillia incisa
Original size:	1062 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	605	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaac
101   gggcaatcct gtgccaaatc ctgttttatg aaaacaaaca aggatttcat
151   aaaccgagaa taaaaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttggct gcattgtgtt agtaaaggaa ttcttccacc
251   gaaacttcag aaaggatgaa ggataaacct atatacatac gtatagtact
301   gaaatactat ctccgaatga ttaatgacga cccgagtctg tattttttta
351   tatttatatg aaaaatgaaa gaattgttgt gaatcgattc caaattgaaa
401   aaagaatcga atattcattg atcaaatcat tcactccacg atagtctgat
451   agatctcttt ttaagaattt attaatcgga cgagaataaa gatagagtcc
501   cattctacat gtcaatatcg acaacaatga aatttatagt aagaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details