JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102157.1
Database:	nt	Go to original record
Description:	Sapindus mukorossi isolate P193 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Sapindus mukorossi
Original size:	1059 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	623	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttatg aaaacaaaac aaacaggggt
151   tcagaaagtg ataaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgtcttcc tttttttggt aaagaaagga aaatgaatcc
251   ttctatcgaa tatcgaaact ccataaagga tgaaggttaa atctatatac
301   actatggata tgaaatgaaa aactatctca aaaataacaa cccaaatctg
351   tatttatgtt aatgaaaaaa aaaaaagcaa aagaattgtt gtgaatcgct
401   tacaagttga agaaagaatc gaatattcat tgatcaaatc atttcctcca
451   taatctggta gatcgtttct tttgaagaac tgattaatcg gacgagaata
501   aagatagagt cccattctac atgccaatat caataccggc aacaatgaaa
551   tttatagtaa gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details