JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102155.1
Database:	nt	Go to original record
Description:	Boehmeria pannosa isolate P188 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Boehmeria pannosa
Original size:	964 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	587	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctaccgag tgataacttt caaattcaga gaaacccggg aattaaaaat
101   gggcaatcct gagccaaatc cgtgtttatg aaaacaaaga agggttcgga
151   aagtggtaaa aaaaaataaa ggataggtgc agagactcaa tggaagttgt
201   tctaacaaat ggagttggct actttgcgtt agtagttagt aaaggaatcc
251   ttccattgaa actccagaaa ggatgaagaa taaatgtata taggtacgga
301   aatactatct ccaaataatt aattacaacc cgaattcgta tttcttttaa
351   ttttcatgaa aattaaaaga attcttgtga ataaattcta agttgaaaaa
401   agatatcaaa tcacttcttc catcaaaatc tgatagattt tttgaagaag
451   tgattaatcg tacgagaata aagatagagt cccattctac atgtcaatat
501   cgacaacaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details