JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102154.1
Database:	nt	Go to original record
Description:	Aphananthe aspera isolate P187 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Aphananthe aspera
Original size:	979 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	610	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cggttttctg aaaacaaaga agggttcaga
151   aggcaataat aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaatggag ttggctatgt tgcgttacga aaggaatcct tccatcgaaa
251   cttcagaaag gatgaagaat aaacctatat acgtatatgt actgaaatac
301   tatctccaaa tgattaatga caacccgaat ccgtatcctt attgcttttc
351   atttttatga aaggtgaaag aattgttgtg aatcaattct aagttaaata
401   agttaaaaaa gaatcgaata ttcattgatc aaatcattta ctccatcaaa
451   atcggataga tcttttgaag aattgattaa tcggacaaga ataaagatag
501   agtcccattc tacatgtcaa tatcgacaac aatgcaattt atagtaatag
551   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details