JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102152.1
Database:	nt	Go to original record
Description:	Actinidia polygama isolate P181 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Actinidia polygama
Original size:	1019 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	591	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaacaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggggttaact gcgttggtag aggaatcctt ccatcgaaac
251   ttcagaaagg atgaaagaga aacctatata catacgcata cgtactgaaa
301   tacttaatca aatgattaat gacggggtat ccgtattttt ttttatgaaa
351   aatggacgaa ttgttgtgaa tcgattcccc attgcataaa gaattgaata
401   tttatttatt gatcaaatca tttactccat agtctgatag atctttttaa
451   gaactgatta atcggacaag aataaagata gagtcccatt ctacatgtca
501   ataccgacaa caatgaaatt gatagtacga ggaaaatccg tcgactttag
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details