JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102151.1
Database:	nt	Go to original record
Description:	Viburnum dilatatum isolate P171 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viburnum dilatatum
Original size:	1037 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	586	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   atgggcaatc ctgagccaaa tcctgttttc cgaaaacaaa caaagaagcg
151   aaaaaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttgactg tgttgcgttg gtagaggaat ccttccatcg aaacttcaga
251   aaggatgaag gataacccta atatacatat acatactgaa atactatatc
301   aaatgatcaa tgacgactcg aatctgtatc tgtcattttt ttctataaaa
351   atagaagaat cgttgtgaag caattccata ttgaagaaag aatctaatat
401   tcattgatca aatcattcac tccatagtct gatagatctt ttgaagaaat
451   gattaatcgg acgagaataa agatagagtc ccattctaca tgtcaatacc
501   gacaacaatg aaatttatag taagaggaaa atccgtcgac tttagaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details