JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102148.1
Database:	nt	Go to original record
Description:	Akebia quinata isolate P163 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Akebia quinata
Original size:	1034 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	589	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaaaaa ggttcagaaa
151   gcgagattaa aaaaataaag gaaggatagg tgcagagact caatggaagc
201   tgttctaacg aatggagttg gctgcgttgc gttcgtagag gaatccttct
251   attgaaactc cagaaaggat gaaggatgaa cctatatacg tacgtatacg
301   tactgaaata tcaaaagatt aatgacgatc aaaatatttt ttttatatga
351   aaaacggaag aattattgtg aatcaatttc aagttgaagg aaaaatcgaa
401   tattcactga tcaaatcagt cattccatag tctgatagat cttttgaaga
451   actgactaat cggacgagaa taaagataga gtcccattct acatgtcaat
501   accgacaata atgaaattta tagtaagagg aaaatccgtc gactttagaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details