JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102147.1
Database:	nt	Go to original record
Description:	Acer mono isolate P162 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Acer pictum subsp. mono
Original size:	1005 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	599	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaataaaac aaagcaaaca
151   agggttcaga aagcgagaaa agggataggt gcagagactc aacggaagct
201   gttctaacaa atggagttga ctgccttttt ttgggaaaga aagggaaatt
251   aatcgaatat cgaaactgca taaaggataa gtttataata cactatggat
301   acaaaatgaa aaattatctc aaaaatgaca acctgaagct gtatttatat
351   taatgaaaaa gaaaacaaaa gaattgggat tccaagttga agaatcgaat
401   attcattgat caaatcattc actccatagt ctggtagatc gtttcttttg
451   aagaacttat tactcagacg agaataaaga tagagtccca ttctacatgt
501   caatataaat accggcaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details