JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102145.1
Database:	nt	Go to original record
Description:	Styrax japonicus isolate P159 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Styrax japonicus
Original size:	1022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	589	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaaatgggca atcctgagcc aaatcctatt tttttaaaac aaagattcag
151   aaagcgaaaa taaaaaagga taggtgcaga gactcaatgg aagctattct
201   aacaaatgga gttgactgcg tcggtatagg aatctttcca tcgaaacttc
251   agaaaggatg aaagagaaac atatatacat atgtactgaa atactatatc
301   aaattatcaa atgattaatg acgacccaaa gctgtatctg tatttttttt
351   tatcaaaaag aattgttgtg aatcgattcc acattgaata aagaattgaa
401   tattcattga tcaaaccatt tactccgcag tctgatagat cttttgaaaa
451   actgattaat cggacaagaa taaagataga gtcccattct acgtgtcaat
501   atcgacaaca atgaaatttt tagtaagagg aaaatccgtc gactttagaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details