JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102144.1
Database:	nt	Go to original record
Description:	Clerodendrum trichotomum isolate P157 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Clerodendrum trichotomum
Original size:	938 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	566	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttgctaag tgatgacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaca aaggtttaaa
151   aaacgaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgctggt agaggaattt ttccatggaa acttcagaaa
251   ggatgaagga taaatggatc tattgaatac tatatcgaat gattaatgat
301   ggcccgaatc tgtatttttt aatatgaaaa atggaagaat tggtgtgaat
351   tgattccaca ttgaagaaaa aatcaaatat tcattcatca aatcattcac
401   tccacagtcc gatagatctt ttcaagatct gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatacc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details