JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102141.1
Database:	nt	Go to original record
Description:	Alangium platanifolium var. macrophyllum isolate P151 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Alangium platanifolium var. macrophyllum
Original size:	987 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	568	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaatca aaaaaggata
151   ggtgcagaga ctcaacggaa gctgttctaa caaatggagt tgactacgtt
201   gcgttgttag aagaatcttt tcatcgaaac ttcagaaagg atgaaggata
251   aaactatata catacgtact gaaatattat atcaaatgat taatgacgac
301   ccgaacctgt atctgtattt tttatatgaa aaatggaaga gttgttgtga
351   atcgattcca aattgaagaa agaatcaaat attcattcat caaatcattc
401   actccatagt ctgatagatc ttttgaagaa ctgattaatc gaacgagaat
451   aaagatagag tcccattcta catgtcaata ccgacaacaa tgaaatttat
501   agtaagagga aaatccgtcg attttataaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details