JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102140.1
Database:	nt	Go to original record
Description:	Veratrum patulum isolate P149 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Veratrum patulum
Original size:	1077 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	625	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggcatggaa
51    acctgctaag tgataacttc caaattcagg gaaaccctgg aaaaaaaagg
101   gggcaatcct gagccaaatc ttgattttga gaaatcaagg gtttaggttt
151   agtttctaaa atcaaactag aataaaaaaa gataggtgca gagactcaat
201   ggaagctgtt ctaacgaatg gagttgacta cgttgcgttg atcgctggaa
251   ttcctctatg gaaattaaag aaaggatggc ccacatatat tttatacata
301   tactttatat atatatatac tgacatatcg atcgattaat catgacccga
351   atccatatta tatattatat aaaatatata tgaaaaagga agagttattg
401   tgaatccatt ccaatcgaag ttaagggaag aatcgaatat tcagtgatca
451   aatcattcat tccaaagtct aatagatctt ttgaaaaagg attcatagga
501   cgagaataaa gagagagtcc cattatacat gtcaataccg acaacaatga
551   aatttatagt aagaggaaaa tccgtcgacc ttagaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details