JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102138.1
Database:	nt	Go to original record
Description:	Elaeagnus umbellata isolate P141 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Elaeagnus umbellata
Original size:	972 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	604	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaataat
101   gggcaatcct gagccaaatc cagttttctg actctgaaaa caaagaaggg
151   ttcagaaagc aataaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg ttgtgttagt aaaggaatcc ttccatccaa
251   acttccgaaa ggatgagatg aagaagaccc tatatatacg atatacgtac
301   tgaaatacta tctgaaaatg attaatgatg gcctgaatct ttttttttta
351   tatatgaaaa atgaaagaat tgttgtgaat cgattcacat ttgaaaaaaa
401   aaatattgat tgatcaaatc atttactcca tctactccat cataatctga
451   cagatctttt gaagaattga ttaatcagac gagaataaag atagagtccc
501   attctacatg tcaatatcga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details