JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102137.1
Database:	nt	Go to original record
Description:	Hepatica insularis isolate P72 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Hepatica nobilis var. japonica
Original size:	837 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	590	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttggttg gattgagcct tggtatggaa
51    acatactaag tgataacttt caaattcaga gaaaccccgg aataaaaaat
101   gggcaatcct gagccaaatc cttttttttt cagaaaacaa acaaaagggc
151   ttcagaaagc aagaataaac aaaaggatag gtgcagagac taaatggaag
201   ctgttctaac gaatagagtt gactgcgttg atcgaggatt ccttctattt
251   aaactacaga aaggatgaac ccgtatacat ataataaaaa aacatcaaag
301   attaatggcc atccaaatct ttattttttt cttatatgat atgaaaaatg
351   gaagaattct tgtgatgtga atcgattcca agtttaagta agaatcaaat
401   attcactgat caaataagtc actctataat ctgatagatc ttttaaagaa
451   ctaattggac gagaataaag atagagtccc attatacatg tcaatatcaa
501   tactgacaaa aatgaaattt atagtaagag gaaaatccgt cgactttaaa
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details