JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ208920.1
Database:	nt	Go to original record
Description:	Caesalpinia pannosa isolate PANNGPL2047 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Erythrostemon pannosus
Original size:	1065 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	589	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcttttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggataaagga tgaacatata tatatgtata
301   ctgaaatatt atttcaattg attagaccag acagacccca aatccctatt
351   ttttaatatt tatatgacaa atgaaagatg tgaatagatt ccaagttgaa
401   gaaagaatca aatgatcaaa tcattcattc catgatagtc tgataaatct
451   tttgaagaac ttattaatcg gacgagaata aagatagagt cccattctac
501   atgtcaatac cgacaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details