JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ208901.1
Database:	nt	Go to original record
Description:	Caesalpinia epifanioi isolate Cepi6 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Erythrostemon epifanioi
Original size:	1053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	595	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcttttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatatgtata
301   ctgaaatctt atttcaattg attagaccag acagaccccc aatctctatt
351   ttttaatatt tatatgacaa atgaaagatg tgaatagatt ccaagttgaa
401   gaaagaatca aatatttatt gatcaaatca ttcattccat gatagtctga
451   taaatctttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaatactga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details