Match details
| Record ID: |
JX434027.1 |
| Database: |
nt |
Go to original record |
| Description: |
GM reference vector pTLH10, partial sequence. |
| Taxon: |
GM reference vector pTLH10 |
| Original size: |
3360 bp |
View other 25 matches for this record
| Method: |
QT-TAX-ZM-005 |
Go to GMOMETHODS record |
| Description: | Quantitative PCR method for detection of maize high-mobility-group gene |
| Target: | maize high-mobility-group |
View other 8 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
3194 |
3272 |
+ |
79 bp |
|
1 ttggactaga aatctcgtgc tgattaattg ttttacgcgt gcgtttgtgt
51 ggattgtagg acaaggctcc ctatgtagc
View other 35 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
23 bp |
0 |
0 |
1 |
23 |
1.00e-10 |
| primerB |
100.0 |
22 bp |
0 |
0 |
79 |
58 |
3.41e-10 |
| probe |
100.0 |
16 bp |
0 |
0 |
56 |
41 |
5.28e-07 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
