JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ208875.1
Database:	nt	Go to original record
Description:	Caesalpinia oyamae isolate TEH9 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Erythrostemon oyamae
Original size:	1077 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	598	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gagttaattg gatagagcct tggtatggaa
51    acttactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcttttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatatatgta
301   tactgaaatc ttatttcaat tgattagacc agacagaccc ccaatctcta
351   ttttttaata tttatatgac aaatgaaaga tgtgaataga ttccaagttg
401   aagaaagaat caaatattta ttgatcaaat cattcattcc atgatagtct
451   gataaatctt ttgaagaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatact gacaacaatg aaatttatag taagaggaaa
551   atccctcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details