JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ208873.1
Database:	nt	Go to original record
Description:	Caesalpinia oyamae isolate TEH4 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Erythrostemon oyamae
Original size:	1070 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	591	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gatagagcct aggtatgcct
51    cttactaagt gagacctttc aaattcagag acccctggaa ttaacaatgg
101   gcaatcctga gccaaatcct gttttccgaa aaccaataag agttcagaaa
151   gggagaataa aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgacatt tcttttcgtt agtaaaggaa ttcttccatc
251   gaaactccag aaaagaaagg atcaaggatg aacatatata tatatgtata
301   ctgaaatctt atttcaattg attagaccag acagaccccc aatctctatt
351   ttttaatatt tatatgacaa atgaaagatg tgaatagatt ccaagttgaa
401   gaaagaatca aatatttatt gatcaaatca ttcattccat gatagtctga
451   taaatctttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg gcaatactga caacaatgaa atttatagga agaggaaaat
551   ccgcgactta gaatcgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details