JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ673259.1
Database:	nt	Go to original record
Description:	Jasminum subhumile psaA-psbB fragment, chloroplast.
Taxon:	Chrysojasminum subhumile
Original size:	36303 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	16481	17044	-	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccccaaacaa aagttcagaa
151   agaaaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagtggacta cgttggtaga ggaatctttc catcgaaact tcagaaaaga
251   tgaaggataa attgtatata ttgaatacta tatcaaagaa tactatatca
301   aatgattaat gacgactcga atctctatct gtattttttt tatatgaaaa
351   atggaagatt gattgcattt tgaagaaaga atcgaatatc cattgatcaa
401   atcattcact ccatagtctg atagatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaataccg gcaacaatga
501   aatttatagt aaaaggaaaa tccgtcgact ttaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details