JRC GMO-Amplicons | EURL GMFF

Record ID:	JX275901.1
Database:	nt	Go to original record
Description:	Dipteryx magnifica voucher L.P.de Queiroz 13912 (HUEFS) tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Dipteryx magnifica
Original size:	847 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	609	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggaaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcata
151   aagcgagagt aaagtaaaaa aggataggtg cagagactca atggaagttg
201   ttcgaacaaa tggagttgac aacatttctt ttcgcgttag gaaaggaatc
251   cttccatcga aattccagaa aggaaaggat caatgataaa tatatatata
301   tacgtatatg tactgaaata ctattttatt tcttaatgaa gactccaaaa
351   atctatattt ttgaattgaa tatttctatc acaaatgaaa gatgtgaatc
401   aattccaagt tgaagaaaga atcgaatatt cattgatcaa atcattcact
451   ccatcatagc ctgatagatc ttttgaagaa ctgattaatc agatgagaat
501   aaagatagag tcccattcta catgttaata ccgacaacaa tgaaatttat
551   agtaaaagga aaatccgtcg actttagaaa tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details