JRC GMO-Amplicons | EURL GMFF

Record ID:	JN591010.1
Database:	nt	Go to original record
Description:	Ligustrum compactum voucher Li 3611 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Ligustrum compactum
Original size:	894 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	549	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaataa aggttcagaa
151   agaaaaaagg ataggtgcag agactcaatg gaagctgttc taacaaatgg
201   agtggactac gttggtagag gaatctttcc atcgaaactt cagaaaggat
251   gaaggataaa cgtatatatt gaatactata tcaaattatt aatgacgact
301   cgaatctcta tctgtatttt tttatatgaa aaatggaaga attgattcca
351   cattgaagaa agaatcgaat attcattgat caaatcattc actccatagt
401   ctgatagatc ttttaaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccattcta catgtcaata ccggcaacaa tgaaatttat agtaaaagga
501   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details