JRC GMO-Amplicons | EURL GMFF

Record ID:	JN226784.1
Database:	nt	Go to original record
Description:	Zanthoxylum ailanthoides isolate P699 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Zanthoxylum ailanthoides
Original size:	1083 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	630	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagccc tagtatagaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aattaaaaat
101   gggcaatcct gagccaaatc ttcttttcca agaacaaaca agggttcaga
151   aagtgaaaag gggagatagg tgcagagact caatggaagt tgttctaaca
201   aatggagttg actgcccctt tttggtaaaa aaaaaagaaa gtcaaatgaa
251   tccttctatc gaatatcgaa actccataaa ggatgaagga taagggtata
301   tagactatgt atacgcaatg aaaaactatc tcaaaaatga caaccgaatc
351   tttatttctt tttatgaaaa agaaaagaat tgttatgaat cgattctaag
401   ttgaagatag aatcgaatac tcattgctca aatcattcac cccatctcca
451   tagtctgatc aatcttttct tttgactttt gaagaactga ttaatcggac
501   gagaataaag atagagtccc attctacatg tcaatatcaa tacgggcaac
551   aatgaaattt atagtaaaag gaaaatccgt cgactttaga aatcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details