JRC GMO-Amplicons | EURL GMFF

Record ID:	JN226780.1
Database:	nt	Go to original record
Description:	Zanthoxylum schinifolium isolate P610 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Zanthoxylum schinifolium
Original size:	1083 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	630	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatagaa
51    acctactaag tgataacttt caaattcaga gaaacccggg aattaaaaat
101   gggcaatcct gagccaaatc ttcttttcca agaacaaaca agggttcaga
151   aagtgaaaag gggagatagg tgcagagact caatggaagt tgttctaaca
201   aatggagttg actgcccctt tttggtaaag aaaaaagaaa gtaaaatgaa
251   tccttctatc gaatatcgaa actccgtaaa ggatgaagga taagggtata
301   tagactatgt atacgcaatg aaaaactatc tcaaaaatga caaccgaatc
351   tttatttttt tttatgaaaa agaaaagaat tgttatgaat cgattctaag
401   ttgaagatag aatcgaatac tcattgctca aatcattcac cccatctcca
451   tagtctgatc catcttttct tttgactttt gaagaactga ttaatcggac
501   gagaataaag atagagtccc attctacatg tcaatatcaa tacgggcaac
551   aatgaaattt atagtaaaag gagaatccgt cgactttaga aatcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details