JRC GMO-Amplicons | EURL GMFF

Record ID:	JN226779.1
Database:	nt	Go to original record
Description:	Schizophragma hydrangeoides isolate P583 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Hydrangea hydrangeoides
Original size:	1059 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	604	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaatcaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaagttc
151   agaaagcgaa aatcaaaaaa ggataggtgc agagactcaa tggaagctat
201   tctaacaaat ggagttgact gccttgcgtt ggtagaggaa tcctttcatc
251   gaaacttcag aaaagatgaa gataaaccta tatacatacg tatacgtact
301   gaaatattat atcaaatgat taaagacgac tcgaatctgt atctgtattt
351   ttatatatga aaaaatggaa gaattgttgg aaatcgattc caaattgaag
401   aaagaatcga atattcattg atcaaatcat tcactccata gtctgataga
451   tctttttttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details