JRC GMO-Amplicons | EURL GMFF

Record ID:	JN226778.1
Database:	nt	Go to original record
Description:	Hydrangea serrata f. buergeri isolate P596 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Hydrangea serrata f. buergeri
Original size:	1046 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	604	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttc caaattcaga gaaaccctgg aattaatcaa
101   aacgggcaat cctgagccaa atcctgtttt ccgaaaacaa gcaaaagttc
151   agaaagctaa aatcaaaaaa ggataggtgc agagactcaa tggaagctat
201   tctaacaaat ggagttgact gccttgcgtt tgcgttggta gaggaatcct
251   ttcatcgaaa cttcggaaag gatgaagata aacctatata catacgtata
301   cgtacttaaa tttgatatca aatgattaaa gacgacccga atctgtattt
351   tgttatatga aaaaatggaa gaattgttgt aaatcgattc caaattgaag
401   aaagaatcga atattcgttg atcaaatcat tcactccata gtctgataga
451   tctttttttt gaagaactga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tggaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details