JRC GMO-Amplicons | EURL GMFF

Record ID:	JN226773.1
Database:	nt	Go to original record
Description:	Hydrangea petiolaris isolate P160 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Hydrangea petiolaris
Original size:	1044 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	602	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccctgg aattaatcaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaagttc
151   agaaagcgaa aatcaaaaaa ggataggtgc agagactcaa tggaagctat
201   tctaacaaat ggagttgact gccttgcgtt ggtagaggaa tcctttcatc
251   gaaacttcag aaaggatgaa gataaaccta tatacatacg catacgtact
301   gaaatattat atcaaatgat taaagacgac ccgaatctgt atctgtattt
351   tttacatgaa aaaatggaag aattgttgta aatcgattcc aaattgaaga
401   aagaatcgaa tattcattga tcaaatcatt cactccatag tctgatagat
451   ctttttttga agaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca acaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details