JRC GMO-Amplicons | EURL GMFF

Record ID:	MK493295.1
Database:	nt	Go to original record
Description:	Lilium pensylvanicum chloroplast, complete genome.
Taxon:	Lilium pensylvanicum
Original size:	152058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44519	45130	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttttaa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagttgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   attcttctat caaaattaca gagaggatgc ccgccctata tacgtatata
301   tacatactga ccatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatatatga aaaatttaag agttattgta aatctattcc
401   atattccaat caaagtttaa ggaagaatcg aatattcagt gatcaaatta
451   ttcattccag agtctgtata gatcttttta aaaactgatt attcggacga
501   gaataaagag agagtcccat tctacgtgtc aataccgaca acaatgaaat
551   ttatagtaaa aggaaaatcc gtcgacttta taagtcgtga gggttcaagt
601   ccctctatcc cc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details