JRC GMO-Amplicons | EURL GMFF

Record ID:	MW238407.1
Database:	nt	Go to original record
Description:	Mazus sp. JY-2020 chloroplast, complete genome.
Taxon:	Mazus sp. JY-2020
Original size:	152388 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47166	47732	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   agtgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaagaaa aggataggtg cagagactca atggaagcta
201   ttctaacaaa tggagttgac tgcattggta gaggaatctt tccatcaaaa
251   cttcagaaag gatgaaggat aaacgtatct attgaatact atatcaatta
301   atgatggccc aaatctgtat ctgtattttt taatctaaaa agtggaagaa
351   ttagtgtgaa ttgattccac attgaagaaa gaatcgaata ttaattcatc
401   aaatcattca ctccatagtc cgacagatct tttaaagaac ttattaatcg
451   gacgagaata aagatagagt cccattctac atgtcaatat tggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga cttttaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details