JRC GMO-Amplicons | EURL GMFF

Record ID:	MW246161.1
Database:	nt	Go to original record
Description:	Chonemorpha megacalyx chloroplast, complete genome.
Taxon:	Chonemorpha megacalyx
Original size:	153862 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48292	48851	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atggaggata tacatatgta ttgaatacta tatcaaatga ttaatgccga
301   cccgaatgaa tctatatttt tttctataaa aaacggaaga attggtgtga
351   ttcgactctt tcttcaatgt ggaatcgaat attcattgat caaacgattc
401   actccatagt ctgtagatcc tcttttcaag aacggattaa tcggacgaga
451   ataaagatag agtcccgtcc tacatgtcaa tgccggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details