JRC GMO-Amplicons | EURL GMFF

Record ID:	CP056063.1
Database:	nt	Go to original record
Description:	Oryza sativa Indica Group cultivar Zhenshan 97 chromosome 12.
Taxon:	Oryza sativa Indica Group
Original size:	27232431 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	10603628	10604242	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaatttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccatgttttg agaaaacaag cggttctcga
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagggaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta aaatgaaaat aggaatgatt atgaaataga aaattcataa
401   tttttttaga attgttgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcatagtttt cgaaatcttt ttaaaagtgg attaatcgga
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttctaagtca tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details