JRC GMO-Amplicons | EURL GMFF

Record ID:	MW123901.1
Database:	nt	Go to original record
Description:	Bougainvillea peruviana voucher 2014052606 SZG chloroplast, complete genome.
Taxon:	Bougainvillea peruviana
Original size:	153966 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47924	48534	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt cttttcaaaa gaaaaggttc
151   agaaatcaag aaaaaaagga taggtgcaga gactcaaagg aagctgttct
201   aacaaatggg gttgactgtg ctgtgttgtt atcagaattc ttccatccaa
251   attccaatcc aaaaaaaaag ggtgaagcat atacgtactc aactatatca
301   aataattaat aacaacctga atcggtattt ttttttctca tttttatata
351   tgaaatgaaa aaatttatat gagaaaaaaa tggaagaatt cttgtgattg
401   tgaatcgatt ccaagttgaa aaaagaatca aatattcatt catcaaacca
451   ttcactccat agtctgatag attttttgaa gaactgatta atcggacgag
501   aatgaagaga gagtccagtt ctacatgtca atactgacaa caatgaaatt
551   tataataaga agaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details