JRC GMO-Amplicons | EURL GMFF

Record ID:	MT312247.1
Database:	nt	Go to original record
Description:	Scutellaria tsinyunensis chloroplast, complete genome.
Taxon:	Scutellaria tsinyunensis
Original size:	152066 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47776	48327	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttag ctgcgttgtt agaggaatcc tttccgcggg aaacttcaga
251   aaggataaac gtatctattg aatactatat caaatgatta atgatggccc
301   gaatctgtat ttttgaatat gaaaaacgga agaatcggtg tgaattgatt
351   ccacattgaa aaaaaaaatg gaatattcat tcatcaaatc attcactcca
401   cggtccaata ggtcttttaa agaactaatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details