JRC GMO-Amplicons | EURL GMFF

Record ID:	KU763487.2
Database:	nt	Go to original record
Description:	Poa fibrifera voucher Peterson et al. 21907 US trnT-trnF intergenic spacer region, partial sequence; chloroplast.
Taxon:	Poa fibrifera
Original size:	1809 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	830	1445	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttctaaaaag aagggcttta tacagctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gagcccatat tataatataa tattggttct
351   ttattctttt ttagaatgaa atagaaatga ttatgaaata aaaaattcat
401   aattttttta gaattattgt gaatccattc caatcgaata tttagtaatc
451   aaatccttca gttcaaagtt ttgaggtctt taaaaaagtg gattaatcgg
501   acgaggacaa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details