JRC GMO-Amplicons | EURL GMFF

Record ID:	MW020353.1
Database:	nt	Go to original record
Description:	Chloris virgata chloroplast, complete genome.
Taxon:	Chloris virgata
Original size:	136065 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47660	48191	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctgttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtgtaatta cgtttaatca cagaacccat acgataatat
251   aggttcttta tttatttttt ataatgaaat taggaatgat tatgaaatag
301   aaaattcata atttttttag aattataatt tttgtgaatc tattccactc
351   gaatattgag taatcaaatc cttcaattca ttgtttttga gatatttaaa
401   aaataggatt aatcggacga ggataaagag agagtcccat tctacatgtc
451   aatactgaca acaatgaaat ttctagtaaa aggaaaatcc gtcgacttta
501   taagtcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details