JRC GMO-Amplicons | EURL GMFF

Record ID:	LR890024.1
Database:	nt	Go to original record
Description:	Musa schizocarpa genome assembly, chromosome: chloro2
Taxon:	Musa schizocarpa
Original size:	206939 bp

View other 1 match for this record

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	173550	174160	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttattttga gaaaacaaag gtttataaaa
151   ctagaatttt aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatggagttg actacgtttt gttggtagca ggaatccgtc tatctaaaaa
251   attacaaaaa gatggtccta tatacctaat acgtatgtat acatactgcc
301   atatcaaacg attaatcatg acccgaatct attatattat aattatatat
351   aattataata tgcaaaattc agagttatta tgaatccatt tcaatggaag
401   ttgaaggaag aatcgaatat tcaattatga aatcattcat tccagagttt
451   gatagatctt tttttgaaaa acggattaat cggacgagaa taaagagaga
501   gtcccattct acatgtcaat accgacaaca acaacaatga aatttatagt
551   aagaggaaaa tccgtcgact ttaaaaaaaa aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details