JRC GMO-Amplicons | EURL GMFF

Record ID:	LR890023.1
Database:	nt	Go to original record
Description:	Musa schizocarpa genome assembly, chromosome: chloro1
Taxon:	Musa schizocarpa
Original size:	269415 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
2	1	162729	163331	-	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttattttga gaaaacaaag gtttataaaa
151   ctagaatttt aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatggagttg actacgtttt gttggtagca ggaatccgtc tataaaaatt
251   acagaaaaga tggtcctata tacctaatac gtatgtatac atactgccat
301   atcaaacgat taatcatgac ccgaatctat tatattataa ttatatataa
351   ttataatatg caaaattcag agttattatg aatccatttc aatggaagtt
401   gaaggaagaa tcgaatattc aattatgaaa tcattcattc cagagttgat
451   agatcttttt tgaaaaaaaa cggattaatc ggacgagaat aaaagagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaaa aaaaatcgtg agggttcaag tcccctatcc
601   cca

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08

Match details