Match details
Record ID:
JX155386.1
Database:
nt
Go to original record
Description:
Synthetic construct neomycin-kanamycin phosphotransferase type III, TrfA transcriptional repressor protein, and phosphinothricin acetyltransferase II genes, complete cds.
Taxon:
synthetic construct
Original size:
8013 bp
View other 12 matches for this record
View other 1579 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
7644
7711
+
68 bp
1 ttgagggtgt tgtggctggt attgcttacg ctggaccttg gaaggctagg
51 aacgcttacg attggaca
View other 10 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
9.83e-10
primerB
100.0
21 bp
0
0
68
48
9.83e-10
probe
90.9
22 bp
2
0
44
23
6.28e-07
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.