JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ180417.1
Database:	nt	Go to original record
Description:	Lycianthes rantonnei isolate RGO 364 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lycianthes rantonnei
Original size:	1930 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	762	1335	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgtaaacaa ataaagattc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg tgttggtaga ggaatctttc catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat tttaaaaaat
351   agaagaattg gtgtgaatcg attctacatt aaagaaagaa tcgaatattc
401   attgatcaaa tcattcactc catagtctga tagatctttt gaagaactga
451   ttaatcggac gagaataaag atagagtccc gttctacatg tcaataccgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details