JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798381.1
Database:	nt	Go to original record
Description:	Meconopsis chelidonifolia voucher Yele tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cathcartia chelidoniifolia
Original size:	744 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	612	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cggttttcag aaaacaaata aggagtttct
151   gaaagcgaga ataaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga ggaatccttc tattcaattg
251   aaattgaaac tccagaaaga atgagggatg aacctatata tgatacgtac
301   gtatacgtac tgaaatataa aagattcgat taatgacgcc ccgaatatgt
351   atttttttac ataaaaaggg gaatcatttt tttgtgaatc gattccaaat
401   tgaagtaaga atcgaatatt cagtgatcaa atcattcact ccatagtctg
451   gtagatcttt tgacgaattg attaatcgga cgagaataaa gatagagtcc
501   cattctacat gtcaatttca ataccgacaa caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details