JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798377.1
Database:	nt	Go to original record
Description:	Meconopsis betonicifolia voucher Cuona3 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Meconopsis betonicifolia
Original size:	825 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	611	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaaatg aagggatttc
151   tgaaagcgag aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaatgaat ggagttgact gcgttggtag aggaatcctt ctattaaatt
251   ttcatttaaa ctccataaag aatgagggat gaacctatat acgatacgta
301   cgtatacgta ctgaaatatc aaagattcta ttaatgccgc cctaaatata
351   aatttttttt acataaaaaa ggaataattt ttgtgaatct attccaagtt
401   gaaggaagaa tagaaaattc agtgatcaaa ccattcactc catagtctga
451   tagatctttt gacgaactga ttaatcggac gagaataaag atagagtccc
501   gttctacatg tcaatatcaa taccgacaac aatgtaattt atagtaagag
551   gaaaatccgt cgactttata aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details