JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798376.1
Database:	nt	Go to original record
Description:	Meconopsis simplicifolia voucher Sejilashan2 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Meconopsis simplicifolia
Original size:	824 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	610	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctagtaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaattaa gggatttctg
151   aaagcgagaa taaaaaaagg ataggtgcag agactcaatg gaagctgtta
201   taatgaatgg agttgactgc gttggtagag gaatccttct attaaatttt
251   aatttaaact ccataaagaa tgagggatga acctatatac gatacgtacg
301   tatacgtact gaaatatcaa agattctatt aatgccgccc taaatataaa
351   ttttttttac ataaaaaaag gaataatttt tgtgaatcta ttccaagttg
401   aaggaagaat agaaaattca gtgatcaaac cattcactcc atagtctgat
451   agatcttttg acgaactgat taatcggacg agaataaaga tagagtcccg
501   ttctacatgt caatatcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttataa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details