JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798335.1
Database:	nt	Go to original record
Description:	Meconopsis integrifolia isolate H16 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Meconopsis integrifolia
Original size:	822 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	609	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaatta agggatttct
151   gaaagcgaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga ggaatccttc tattaaattt
251   taattgaaat tccataaaga atgagggatg aacctatata cgatacgtac
301   gtatacgtac tgaaatatca aagattctat taatgccgcc ctaaatatat
351   atttttttac ataaaaaagg aataattttt gtgaatctat tccaagttga
401   aggaagaata gaaaattcag tgatcaaacc attcactcca tagtctgata
451   gatcttttga cgaactgatt aatcggacga gaataaagat agagtcccgt
501   tctacatgtc aatatcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttataaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details