JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798333.1
Database:	nt	Go to original record
Description:	Meconopsis integrifolia isolate H12 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Meconopsis integrifolia
Original size:	825 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	612	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaaaaa attaagggat
151   ttctgaaagc gagaataaaa aggataggtg cagagactca atggaagctg
201   ttctaatgaa tggagttgac tgcgttggta gaggaatcct tctattaaat
251   tttaatttaa attccataaa gaatgaggga tgaacctata tacgatacgt
301   acgtatacgt actgaaatat caaagattct attaatgccg ccctaaatat
351   atattttttt acataaaaaa aggaataatt tttgtgaatc tattccaagt
401   tgaaggaaga atagaaaatt cagtgatcaa accattcact ccatagtctg
451   atagatcttt tgacgaactg attaatcgga cgagaataaa gatagagtcc
501   cgttctacat gtcaatatca ataccgacaa caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details