JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ798324.1
Database:	nt	Go to original record
Description:	Meconopsis integrifolia isolate H1 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Meconopsis integrifolia
Original size:	825 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	12	611	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaagatta agggatttct
151   gaaagcgaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga ggaatccttc gattaaattt
251   taatttaaac tccataaaga atgagggatg aacctatata cgatacgtac
301   gtatacgtac tgaaatatca aagattctat taatgccgcc ctaaatataa
351   aaatttttta cataaaaaaa gtaataattt ttatgaatct attccaagtt
401   gaaggaagaa tagaaaattc agtgatcaaa ccattcactc catagtctga
451   tagatctttt gacgaactga ttaatcggac gagaataaag atagagtccc
501   gttctacatg tcaatatcaa taccgacaac aatgaaattt atagtaagag
551   gaaaatccgt cgactttata aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details