JRC GMO-Amplicons | EURL GMFF

Record ID:	JN030974.1
Database:	nt	Go to original record
Description:	Poa supina isolate S2 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa supina
Original size:	1820 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	802	1417	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtgtaat tccttctaaa
251   ttcgaaaaag aagggcttta tacatctaat aaacacgtat cgatactgac
301   atagcaaacg attaatcaca gaacccatat tataatattg gttctttatt
351   cttttttaga atgaaataga aatgattatg aaataaaaaa ttctgcattt
401   ttttagaatt attatattgt gaatccattc cactcgaata tttagtaatc
451   aaatccttca attcaaagtt ttgaggtctt taaaaaagtg gattaatcgg
501   acgaggacaa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtt gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details