Match details

Record ID: JQ322789.1
Database: nt Go to original record
Description: Proboscidea triloba subsp. triloba tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Proboscidea triloba subsp. triloba
Original size: 1270 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 304 867 + 564 bp

Amplicon: 564 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgaag gataaacgta tctattgaat actatatcaa atgattaatg
301   atggcccgaa tctgtatctg tattttttta tatgaaaaat ggaagaattg
351   gtgtgaattg attccacatt gaagaaagaa tcgaatattc attcatcaaa
401   tcattcactc catagtccga tagatctttt aaagaactga ttaatcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt aaaaaatcgt gagggttcaa
551   gtccctctat cccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.54e-08
primerB 100.0 20 bp 0 0 564 545 2.54e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.